5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format. For ds DNA can be used kits below: Quant-iT™ PicoGreen ® dsDNA Assay Kit (Invitrogen P7589); Qubit® dsDNA Broad Range Assay Kit (Invitrogen Q32850);. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). 2. • Specific for dsDNA in the presence of RNA. Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. For Research Use. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. • Quant-iT dsDNA Assay Kit, high sensitivity (Cat. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. A 96 plate well was filled with 100 µl of dye and 100 µl of DNA, a 384 well with 10 µl of each solution. i) 110 sample x 150uL =16. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. stranded DNA (dsDNA). The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5. The Quant-iTTM PicoGreen® dsDNA Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures. 7 Q33231 6 1,000 41. 线，利用这一曲线并根据经验检测样本的浓度。Qubit dsDNA HS Assay可用于测量DNA浓度在10pg/µL 至100ng/µL 之间 的样本。超过此浓度范围的样本必须经过稀释然后进行重新 测试，以确保它们的结果落在参考标准的线性范围内。Qubit dsDNA HS Assay具有高度的准确. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. Quantitation Range. • Specific for dsDNA in the presence of RNA. Serial dilutions were made to give appropriate cell, seeding numbers. Product Description. Quantitation using Quant-iT Assay Kits. Quant It Picogreen Dsdna Assay Kit Reagents, supplied by Thermo Fisher, used in various techniques. were treated with standard-of-care chemotherapy in vitro followed by cfDNA quantification via a PicoGreen dsDNA assay. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. This kit contains. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. • Specific for dsDNA in the presence of RNA. 2 – 100 ng. • Specific for dsDNA in the presence of RNA. Rapidly detect as little as 0. Calibrator MPO concentration was quantified with a commercially available assay (Human Myeloperoxidase Quantikine ELISA Kit, Bio-Techne, Minneapolis, MN) according to the manufacturer’s recommendations. 0 fluorometer according to the manufacturer’s protocols; a. low-range. 21 DiB) [22]. PicoGreen® Assay Supplies Equipment: − NanoDrop 3300 Fluorospectrometer − 2uL pipettor (low retention nuclease free pipette tips) Materials:Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). no. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitlimit consume only 2 picograms of dsDNA per measure-ment. The normalized DNA was aliquoted into multiple tubes at 50µL volume. Unit Size. Description. According to the obtained results, the assay presents a linear correlation in a wide range of 0. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. 5ul of 12uM primer diluted in ClontechPicoGreen is highly linear (R 2 ≥ 0. Optimized for fluorescence microplate readers. 8. Quantitation Range. Quantify nucleic acids and proteins using four user-selectable fluorescent. 5. 25 pg/uL 的 dsDNA。 该定量试剂盒在三个数量级内呈线性，几乎无序列依赖性，可让您准确测定多种来源的 DNA，包括基因组 DNA、病毒 DNA、小提 DNA 或 PCR 扩. 005% Triton X-100, Tween-20, or Brij. VIEWS. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. 1–100 ng: Ex / Em (nm) 502 / 523: 510 / 527: 502 / 523: Components: dsDNA HS fluorescent reagent, dilution buffer and standards RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. For example, if the amount of the linear dsDNA library available is 20 ng, the linear dsDNA library input should be 10 ng, and the linear dsDNA library concentration should be at least 0. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Materials: 1. Barcoded amplicons were pooled in. Bioz Stars score: 86/100, based on 1 PubMed citations. Price from $9. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. Begin typing to search, use arrow keys to navigate, Enter to select. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Cloning, expression and proteins purification. Thermo Fisher quant it picogreen dsdna quantitation assay kit. dsDNA standards were incubated in cuvettes and measured using a Varian Cary Eclipse. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The reagent must be completely thawed before using it by bringing it to room temperature. The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. DNA samples with a concentration determined by 260 nm absorbance higher than 100 ng/mL were diluted. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. 10. BR reagent (Component A) 1. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. . Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. in the assay in order to reduce waste and still achieve comparable results. Fluorometer. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. 0). quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Rapidly detect as little as 0. The Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. Lambda DNA was used to generate the. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. 2. The Quant-iT™ PicoGreen® dsDNA reagent is a proprietary, asymmetrical cyanine dye. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Three kits covering a wide range of dsDNA concentrations. Q33130) Metabolic (cholesterol) • Amplex Red Cholesterol Assay Kit (Cat. Got it. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). Rapidly detect as little as 0. The PicoGreen reagent was freshly prepared by diluting 15 μL reagent with 2 mL of TE buffer (~ 133X) and stored in the dark for no longer than 2 hours. Similar. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. Prepared DNA concentration of the samples was 250 ng/ mL. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Each Helixyte™ assay kit is specially formulated with a target-selective DNA. 50 pg to 2 µg. The reagent Quant-iT PicoGreen was added to all wells and the samples were incubated for 5 min at room. 2 The input amount of linear dsDNA library is based on the available linear dsDNA (see Table 3). Using this data as a starting point, the optimal conditions (e. Unit Size. No. 5 µL of each extracted sample was used for Picogreen analysis. 5mL of TE x 5uL PicoReagent. An aqueous working solution of PG reagent was freshly prepared as a 1:200 dilution of the concentrated DMSO solution to 1x TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 7. This sensitivity exceeds that achieved with the Hoechst 33258. DNA standard curve. (The same tips can be used. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 99 to $1999. • Suitable for use in 96- and 384-well plate formats. • Specific for dsDNA in the presence of RNA. Genomic DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 5 ng/µL. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). • Suitable for use in 96- and 384-well plate formats. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 5 ng/ml of dsDNA were detected with minimal effect in the presence of ssDNA, RNA, and free nucleotides, which is comparable with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent. Picogreen kinetic assay was based on polymerase activity of SARS nsp12 in complex with nsp7L8, which. to each well. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The micrococcal nuclease treatment used for fragmentation in some selected ChIP methods will generate. Quant-it Picogreen® dsDNA assay kit (ref P11496) was obtained from Thermofisher scientific. a)Use 110 sample number per plate. Bioz Stars score: 86/100, based on 1 PubMed citations. Rapidly detect as little as 0. 3. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). 2023-11. 10. P7589. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Unit Size. . The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. Description. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). Rapidly detect as little as 0. This assay is designed for use with fluorescence microplate readers. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. 3. Rapidly detect as little as 0. 2 to 100 ng) Assay Kit were used with a Qubit 3. The fluorescence spectra of dsDNA stained with PicoGreen when in solution equimolar concentration of RNA was also present ([DNA]/[RNA] = 1, red lines in figure 4) did notObtenga mediciones precisas de la concentración de ADNbc en un amplio rango dinámico con los kits de ensayo de ADNbc y reactivos de ADNbc Quant-iT Picogreen. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quant-iT RNA BR (Broad Range) Assay Kit. In addition, the assay failed to detect in vitro generated MPO-DNA complexes when spiked into plasma. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Free dye. • Suitable for use in 96- and 384-well plate formats. Description. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. 反应次数. Bioz Stars score: 99/100, based on 1 PubMed citations. • Specific for dsDNA in the presence of RNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 0. 995 mL TE for the . Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. After a 5-minute. Rapidly detect as little as 0. The tubes were. Unit Size. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Catalog number: P7581. Abstract. 5) prepared from 20x. UV흡광 결과보다 민감도 우수. Bioz Stars score: 86/100, based on 1 PubMed citations. The PicoGreen is sticking to the sides of the cuvette or assay plate. 05 to consider statistical significance. Figure 8. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). The dynamic range of this assay in microplate format, as stated in the product manual, is from 250 pg/mL to 1000 ng/mL with a single dye concentration. The AccuBlue® Broad Range RNA Quantitation assay has a linear range of 5-1000 ng RNA in a fluorescent plate reader, a wider range than similar assays. assay. P11495. This protocol is a simplified and condensed version of. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. PicoGreen dsDNA quantitation reagents are. Plasma levels. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Quant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. クイックオーダー. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for each cell line of interest. Therefore, fluorescence spectroscopy using PicoGreen is more useful than UV spectroscopy for evaluating the template activity of DNA for PCR. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. Showing 4 of 4. 1 mL kit. Quant-iT PicoGreen dsDNA Reagent. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Figure 1. Picogreen assays are compatible with most microplate readers and fluorometers. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume). 1X dsDNA assay (%) 100 17. For a standard. P7589. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for. Product Type. The use of a serial dilution of dsDNA standards whileThe quantity of ChIP DNA should first be determined with the Quant-iT™ PicoGreen dsDNA Reagent Kit (Invitrogen). 4 mL of prewarmed to 37°C, TE Buffer Cat# D001 or other qualified TE buffer. Quant-iT dsDNA BR buffer (Component B) 250 ml c. 9, 10 The Pico-Green assay is sensitive to 25 pg dsDNA/mL, which is 400-fold more sensitive than Hoechst 33258 dye. Instead of 2 mL, only 1 mL measurement volume was used. Average 86 stars, based on 1. Technical Support Customer Service. The assay is linear over three orders of magnitude and has little sequence. Catalog Number. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. A comparison of reagents for detecting and quantitating proteins in solution—Table 9. 5 Q32851 15. Picogreen assays are compatible with most microplate readers and fluorometers. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 100 ng/µL of Lamba (λ) DNA, sheared by intensive. Fluorescence was measured at. The assay is linear over three orders of magnitude and has little sequence dependence. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Figure 2. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Q33120). Supplier: Invitrogen™ P11496. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The stock DNA and the corresponding 1:5 diluted DNA derived from FFPE tissue was quantified by using Quant-iT™ PicoGreen dsDNA Assay kit (Thermo Fisher Scientific) as follows: 2 μl of sample DNA were diluted 1:5 vol/vol in 1× TE, containing 1:100 diluted PicoGreen reagent in a final reaction volume of 40 μl per reaction. Picogreen assays are compatible with most microplate readers and fluorometers. 2 ng to 100 ng of DNA in the assay tube. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Rapidly detect as little as 0. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. In this experiment we followed the instructions of the Quant-iT™ PicoGreen dsDNA Assay Kit to compare setup on the Matrix Hydra DT and Matrix PlateMate 2x2 to manual setup and then reduced the total volume from 210 µl to 105 µl on both instruments. Both fluorescent assays were performed with a Qubit fluorometer. using a PicoGreen assay by the company life technologies (Quant-iT™ PicoGreen dsDNA Reagent and Kits). When 5–500 pg salmon dsDNA or 3–1000 pg control dsDNA (calf thymus dsDNA standard) is added to AccuClear a linear response is observed (Fig. High tolerance for contaminants. This protocol is a simplified and condensed version of. An Agilent 2100 Bioanalyzer (Agilent) was used to compare library quality using a High Sensitivity DNA Kit (Agilent), and the purified library was quantified using a Quant-iT PicoGreen dsDNA assay kit (Invitrogen). Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Figure 1. P11495. • Specific for dsDNA in the presence of RNA. assay. The Quant-iT™ PicoGreen® DNA assay is the oldest assay and the most general-purpose. • Suitable for use in 96- and 384-well plate formats. • Suitable for use in 96- and 384-well plate formats. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. Bioz Stars score: 86/100, based on 1 PubMed citations. Prepare the assay. Determine the DNA concentration on the basis of absorbance at 260 nm (A 260) in a cuvette with a 1 cm pathlength; an A 260 of 0. g. 12. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quant-iT PicoGreen dsDNA Assay Kit. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Easy to use UV absorbance alternative assay that can be. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. Natural cures with herbs are. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Picogreen assays are compatible with most microplate readers and fluorometers. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. Accuracy of the Qubit dsDNA HS assay compared to the Quant-iT PicoGreen dsDNA assay plotted on two different types of curves. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. Quant-iT PicoGreen dsDNA Assay Kit. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. Quant-iT PicoGreen dsDNA kit by Thermo Scientific Catalog #: P7589 2Prepare 1XTE buffer from 20X stock solution using nuclease-free water: will need 200 μl/well (for. 1. 04 corresponds to 2 μ g/mL dsDNA solution. DNA standards were prepared from concentrated stock herring sperm dsDNA (Sigma, PN-A3294) in TE buffer (tris-EDTA, pH 7. Unlike many other DNA quantitation methods, PicoGreen is minimally affected by the presence of lipid, protein, carbohydrates and other cellular. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Triplicate 10 μL samples of λ DNA (Ο), E. The DeNovix dsDNA High. DNA was quantified by the Quant-it PicoGreen dsDNA Assay Kit (Invitrogen Life technologies, UK) according to the manufacturer’s protocol. 3ng/mL，DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Quant-iT™ PicoGreen dsDNA Assay Kit (Life Technologies; Cat. (An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. quant it picogreen dsdna assay kit reagents. A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Revised: 10–June–2008 | MP 07581 Table 1. 2 ng to 100 ng of DNA in the assay tube. Rapidly detect as little as 0. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM 製品番号（カタログ番号）： P11495. 2K. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. Catalog Number. 3. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Table 2 summarizes results of the. Thermo Fisher quant it picogreen dsdnaassay kit Quant It Picogreen Dsdnaassay Kit, supplied by Thermo Fisher, used in various techniques. Product Type. The differences in mechanical testing, cell proliferation in PrestoBlue™ and Quant-iT™ Picogreen™ dsDNA Assay were evaluated using t-test and one-way ANOVA, followed by Tukey’s post-test with α = 0. Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Calibrator MPO. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2. 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). If your are using plastic cuvettes or assay plates, reduce this problem by including 0.